The Team Bath star heads-up a 12-strong Roses squad for the tournament, which takes place from 12-21 July.
Guthrie first took on the role last year, filling in for regular skipper Ama Agbeze, but has now been chosen to lead England in their first home World Cup since 1995.
Since leaving Jersey to join Bath as a teenager, Guthrie has grown into one of the world's best players, and a leading star in her position.
The 29-year-old has 90 caps to her name and helped the Roses win an historic first Commonwealth gold in Australia in 2018.
England finished third in the last two World Cups, in 2011 and 2015, and achieved their best result in 1975 when they were runners-up.
Coach Tracey Neville "It is fantastic to see the versatility we have amongst the final 12, plus the emergence of two outstanding leaders in Serena Guthrie as captain and Jade Clarke as vice-captain. Not only are they world class players but together they have set the standard within our Roses programme both on and off the court. Our final 12 have shown that they’re ready to perform and want to win. I’m excited to see these players compete in a home World Cup!"